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Insights of Microbial Pullulan Production: A Bioprocess Engineer Assessment

[ Vol. 7 , Issue. 4 ]


Parul and Kashyap Kumar Dubey*   Pages 262 - 272 ( 11 )


Background: Pullulan is a neutral exopolysaccharide produced aerobically by a yeast-like fungus Aureobasidium pullulans. The distinct linkage of α (1 → 4) & α (1→ 6) in pullulan impart its nonimmunogenic, non-mutagenic and non-cancerous traits. These characteristics are being exploited in food, health care, pharmacy, lithography, cosmetics. During the last four decades, pullulan has had a breakthrough in diverse fields including gene delivery, wound healing, nano-technology, diagnostics and also in human reproductive health.

Objective: The present review focused on food grade pullulan, its derivatives, and their applications. Methods: Deviations in biosynthesis and downstream processing of food grade pullulan have been reviewed in detail.

Results: Pullulan can be used for a variety of applications. The food applications of pullulan powder and film have been thoroughly validated. Pullulan film, being carbon neutral, sustainable, biodegradable and palatable, provides a superior substitute to conventional petrochemical polythene films. Pullulan films could be modified to add a variety of special features. These formulations have clearly demonstrated the significance of pullulan, and its potential for more applications.


Aureobasidium pullulans, pullulan, pullulanase, food grade, exopolysaccharide, fungus.


Microbial Process Development Laboratory, University Institute of Engineering and Technology, Maharishi Dayanand University, Rohtak-124001, Haryana, Department of Biotechnology, Central University of Haryana, Mahendergarh- 123031, Haryana

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