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Isolation, Screening and Antimicrobial Activity of Aeromonas hydrophila from Spoiled Vegetables and Biochemical Analysis of Estuarine Microbial Sediments

Author(s):

Swathi Kumar , Murali Raju , Ramachandhiran Duraisamy and Vinothkumar Veerasamy *   Pages 1 - 8 ( 8 )

Abstract:


Background: The Aeromonas hydrophila is a heterotrophic, Gram-negative, rod-shaped, facultative anaerobic, nonspore forming bacteria that are autochthonous and widely dispersed in marine environments. In this work design to investigate the screening of Aeromonas hydrophila from spoiled vegetable and three different estuarine sediments sample were collected from different estuaries of Bay of Bengal (Vedharanyam, Parangipettai and Pichavaram, Tamilnadu, India) for enzymes and antimicrobial activities.

Objective: Isolation, enzyme screening, antimicrobial activity of Aeromonas hydrophila from spoiled vegetables and three different estuarine microbial sediments sample were used to analysis the biochemical and enzymatic analysis.

Results: The purified isolate of Aeromonas hydrophila strain produces good antimicrobial activity against Aspergillus niger, Candida albicans, Staphylococus, Klebsiella and pseudomonas species.

Methods: The bioactive compound produced by this strain was purified by thin layer chromatography.

Conclusion: These isolates producing amylase, protease, lipase and gelatinase enzymes, which are commercially very important and used in many industries and other biochemical sectors.

Keywords:

Antimicrobial activity, Aeromonas hydrophila, Aspergillus niger, Candida albicans, Staphylococus, Protease

Affiliation:

Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, 608002, Tamilnadu, Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, 608002, Tamilnadu, Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, 608002, Tamilnadu, Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, 608002, Tamilnadu



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