Vivek Chauhan and Shamsher S. kanwar* Pages 1 - 12 ( 12 )
Synthetic dyes find usage in multiple industries such as paper, textile, food, plastic and pharmaceuticals. On their release as industrial effluent into the environment, majority of them affect aquatic and surrounding non-aquatic life due to their carcinogenic nature. So their proper discharge and economical treatment is a matter of great concern. In this context, many enzymes have been found to have a positive effect on dye degradation. Peroxidase is one such enzyme which causes dye degradation either by precipitation of chemical structure of aromatic dyes or by opening up their aromatic ring structure. The current paper focuses on major impacts of the industrial dyes on the surrounding environment and also on exploring the use of bacterial peroxidases as alternative dye degradation compound. Bacterial peroxidase was extracted from bacillus strain BTS-P5, a strain isolated from soil samples. Various parameters were optimized for degradation of 10 [Bismark Brown R (BBR), Bromophenol Blue (BB), Rhodamine B (RB), Bismark Brown Y (BBY), Direct Violet 21 (DV), Basic Fuchsin (BF), Coomassie Brilliant Blue (CBBG), Congo Red (CR), Direct Black 154 (DB) and Methylene Blue (MB)] major industrially important dyes by the enzymes. Basic Fuchsion (BF) showed maximum degradation of about 95% while the Rhodamine B (RB) degraded by only 12%. Out of 10 dyes, 8 dyes showed degradation over 50%.
Peroxidase, Industrial Dyes, Biodegradation, Pollution, Wastewater management, Environment
Department of Biotechnology, Himachal Pradesh University summerhill, Department of Biotechnology, Himachal Pradesh University summerhill