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Standardization Using Spectroscopic Techniques (UV, NMR, FTIR, HPTLC, Mass) and Pharmacognostic Evaluation of the Roots of Selinum vaginatum: A Rare Himalayan Plant of the Rohtang Region


Nikita Saraswat*, Neetu Sachan and Phool Chandra   Pages 1 - 5 ( 5 )


Selinum Vaginatum is an endemic Indian plant present on high altitude and is known as “Bhootkeshi,” by the locals and belongs to family Umbellifercae. Selinum Vaginatum has been used traditionally for curing seizures, epilepsy, mental illness and hysteria. It is a novel work as the plant is rarely found and shows medicinal values but no quality control work has been performed. The aim of this paper is quality control, physicochemical analysis and standardization of the roots of Selinum Vaginatum for determining its quality and purity. The organoleptic characteristics, physichochemical tests, phyto-chemical analysis, fluorescence analysis, TLC, UV, FTIR, NMR and Electrospray Ionisation - Mass spectroscopy, HPLC etc. other parameters were studied for the standardization of the test drug. The total ash values, swelling index, foaming index, moisture content, alcohol and water soluble extractive values were calculated. The TLC profile of Selinum Vaginatum was observed whereas the UV, FTIR, HPLC, NMR and Mass spectroscopies of the plant were also performed. The microscopic results showed presence of schizogenous canals present in abundance, cork cells, oil globules were seen, medullary rays and pith was absent and around 10-17 layers of cells were seen. The presence of active compounds like phenols, terpenoids, alkaloids, coumarins, essential oils, tannins, saponins, carbohydrates etc. were reported by the phytochemical tests, TLC, UV, NMR, HPLC, Mass and FTIR spectroscopy methods. These compounds can be studied in detailed for understanding the potential pharmacological benefits and decoding the traditional uses. The spectroscopic analysis and the physicochemical tests have shown the presence of phenols, esters, organic halogens, carbohydrate, carboxylic acids, alcohols, ethers, glycosides which must be responsible for the medicinal properties of the plant. This study has provided a set of information which will be useful for identification of fresh batches of the roots of Selinum Vaginatum and this can be used as a reference set of information for accessing the purity, quality and identification and analysis of authenticity of the plant roots. The spectroscopic techniques have also reported the presence of active compounds which can be studied for understanding and identifying the pharmacological actions of the rhizomatous roots of S. Vaginatum. All the information collected by research on the roots of S. Vaginatum will help in identification of crude drugs and also in development of standard for the drug.


Selinum Vaginatum, Spectroscopy, Quality control, Phyto-chemical analysis, Ethno-medicine, indigenous medicine, Himalayan medicine, Mass spectroscopy, FTIR, UV, HPLC, NMR


Department of Pharmacy, Pranveer Singh Institute of Technology, School Of Pharmceutical Sciences, IFTM University, Department of Pharmacy, School of Pharmaceutical Sciences, IFTM University, Moradabad

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