Ankit Agrawal, Anjana Sharma and Narmada Prasad Shukla Pages 1 - 7 ( 7 )
Background: Artemisia annua is well known for its anti-malarial bio-active compound artemisinin. Development of elite planting material of A. annua and its agro-technology can fulfill the requirement of Artemisinin based Combination Therapy (ACT) dosages worldwide.
Objectives: To develop an efficient in-vitro propagation protocol for A. annua and assess the field performance of in-vitro propagated plants for their growth and artemisinin yield.
Methods: The in-vitro propagation protocol of A. annua was developed using nodal segment in four steps viz: initiation, multiplication, rooting and hardening. In-vitro propagated plants were transplanted with open pollinated seed raised plants in an experimental field trial having soil supplementation of Farm Yard Manure (FYM), vermicompost and NPK.
Result: Maximum 92% shoots were initiated in Murashige and Skoog medium (MS) with 0.44 µM 6-benzyl aminopurine (BA) and highest 281.33 ± 09.75 micro-shoots/inoculum obtained in MS with 15.54 µM BA. Maximum number of roots were found in MS with 100 mg/L activated charcoal while 78.20% plants survived in sand: soil: vermicompost (1:1:1) mixture. Highest dry leaf yield (6.37 t/ha) was observed in in-vitro propagated plants grown with vermicompost, while highest artemisinin content (1.11 ± 0.10) and artemisinin yield (65.05 kg/ha) was found in the in-vitro propagated plants grown with FYM after 120 days of transplantation.
Conclusion: This study reports enhanced artemisinin yield through in-vitro propagated plants of A. annua and its cultivation using organic soil supplement inputs. This would leads to increase the production of artemisinin yield and reduce the demand of Artemisinin based Combination Therapy (ACT).
Micropropagation, HPTLC, Farm Yard Manure (FYM), Vermicompost, Agriculture, Medicinal plant
International Institute of Waste Management, R&D, Bhopal, Madhya Pradesh, R. D. University Department of Post Graduate Studies and Research in Biological Sciences, MP Pollution Control Board